Division of Clinical Biochemistry
Interim Head: Ludger Hengst

Protein Analysis Group
Our group concentrates on the development of rapid and efficient methods for the separation and characterization of proteins and their post-translational modifications with closest attention to the family of histone and HMG proteins. Histone analysis  was always a driving force behind the biological research in this field, because it constantly provided a better insight into the complexity of this protein family. It is precisely this complexity of histones that, because of their varied modifications and their interplay, today gives us new information for such important biochemical processes as, for example, the activation and repression of genes. Because of the intricacy of histones it is not amazing that an entire repertoire of analytical methods is used for their separation. For this purpose a set of separation methods based on capillary electrophoresis (CE), reversed-phase chromatography, hydrophilic interaction liquid chromatography (HILIC) and mass spectrometry was developed in our lab.

Group members are also allocated to the Protein Micro-Analysis Facility. The protein facility established at Innsbruck Medical University is dedicated to provide investigators with equipment, expertise and custom services for the detection, characterization and quantification of proteins and peptides on a recharge basis. The facility maintains a suite of  state of the art instrumentation including a MALDI TOF/TOF 4800 plus Analyzer (Applied Biosystems), a Hybrid FT-Mass Spectrometer LTQ Orbitrap XL (ThermoScientific), a Procise 492 Protein Sequencer (Applied Biosystems), Nano-LC Gradient Systems UltiMate 3000 (Dionex), a Probot Microfraction Collector (LC-Packings) for on-line MALDI target preparations. Various Capillary Electrophoresis and HPLC Systems and, in addition,  a Solaar M6 Dual Zeeman Spectrometer (ThermoScientific) for trace element analysis are operated in the facility.

Homepage Protein Micro-Analysis Facility:
http://www.i-med.ac.at/iftz/zentrale_gruppen/proteinfacility/